Disentangling subpopulations in single-molecule FRET and ALEX experiments with photon distribution analysis.

@article{Tomov2012DisentanglingSI,
  title={Disentangling subpopulations in single-molecule FRET and ALEX experiments with photon distribution analysis.},
  author={Toma E Tomov and Roman Tsukanov and Rula Masoud and Miran Liber and Noa Plavner and Eyal Nir},
  journal={Biophysical journal},
  year={2012},
  volume={102 5},
  pages={1163-73}
}
Among the advantages of the single-molecule approach when used to study biomolecular structural dynamics and interaction is its ability to distinguish between and independently observe minor subpopulations. In a single-molecule Förster resonance energy transfer (FRET) and alternating laser excitation diffusion experiment, the various populations are apparent in the resultant histograms. However, because histograms are calculated based on the per-burst mean FRET and stoichiometry ratio and not… CONTINUE READING

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