Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study

  title={Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study},
  author={Xavier Libert and Ann Packeu and Fabrice Bureau and Nancy H. C. Roosens and Sigrid C. J. De Keersmaecker},
  journal={BMC Microbiology},
BackgroundIndoor air pollution caused by fungal contamination is suspected to have a public health impact. Monitoring of the composition of the indoor airborne fungal contaminants is therefore important. To avoid problems linked to culture-dependent protocols, molecular methods are increasingly being proposed as an alternative. Among these molecular methods, the polymerase chain reaction (PCR) and the real-time PCR are the most frequently used tools for indoor fungal detection. However, even if… 
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Rapid and accurate identification of black aspergilli from grapes using high-resolution melting (HRM) analysis.
This is the first report on the development of an HRM-PCR assay for the identification of black Aspergilli species in grape samples and it was able to identify A. niger, A. ibericus and A. japonicus in Grape samples artificially inoculated with conidia of these fungi.
Development of high-resolution melting PCR (HRM-PCR) assay to identify native fungal species associated with the wheat endosphere
This work describes a fast, simple, and low-cost method based on high-resolution melting PCR (HRM-PCR) for the identification and differentiation of wheat endogenous fungal isolates and revealed that this method can allow large-scale screening of cultured samples.
Spectral Grouping of Nominally Aspergillus versicolor Microbial-Collection Deposits by MALDI-TOF MS
This study demonstrates the clear resolving power of MALDI-TOF MS when applied to samples deposited in historical microbial collections, and identifies six clear spectral-linkage groups along with a group of ten spectrally-unique samples.
Identification of opportunistic and nonopportunistic Exophiala species using high resolution melting analysis.
The developed HRM assay can be used to ascertain the identity of Exophiala species, which may differ in clinical significance, with high accuracy, and its application to identify species directly in clinical samples and/or environmental niches may be possible in the future.
Multiplex detection of five common respiratory pathogens from bronchoalveolar lavages using high resolution melting curve analysis
The MHRM assay offers a simple and fast alternative to culture approach for simultaneous detection of five major bacterial lower respiratory tract infection pathogens and can help clinicians initiate prompt and appropriate antimicrobial treatment, towards reducing the morbidity and mortality of severe respiratory infections.
Characterization and proliferation capacity of potentially pathogenic fungi in marine and freshwater fish commercial feeds
It has been established that high levels of aflatoxins in feeds can cause alterations in fish growth as well as the development of cancerous tumors in the liver, in addition to enhancing mortality.


Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air
A SYBR® green real-time PCR-based assay for the specific detection and identification of Aspergillus versicolor, which is frequently observed in indoor environment and known to be allergenic, is developed.
Rapid Detection and Identification of Aspergillus from Lower Respiratory Tract Specimens by Use of a Combined Probe–High-Resolution Melting Analysis
A real-time PCR-based method that combined melting temperature calling analysis of a specific probe with high-resolution melting analysis of the full amplicon shows 100% sensitivity and specificity and constitutes an efficient alternative for identification of Aspergillus in lower respiratory tract samples.
Molecular profiling of fungal communities in moisture damaged buildings before and after remediation - a comparison of culture-dependent and culture-independent methods
The sequencing-based method proved indispensable for describing the true fungal diversity in indoor environments and was complicated by the wide natural diversity in the dust samples, the incomplete knowledge of material-associated fungi fungi and the semiquantitative nature of sequencing based methods.
Detection of invasive fungal pathogens by real-time PCR and high-resolution melting analysis.
This technique is not adaptable as a general identification method, but it is highly useful when certain fungal species are to be expected in clinical samples.
Rapid Identification of Medically Important Candida Isolates Using High Resolution Melting Analysis
HRMA as a simple, rapid and inexpensive tool was shown to be useful in identifying a wide spectrum of clinically important Candida species and may complement the current clinical diagnostic approach based on commercially available biochemical kits.
Development of a fungus-specific PCR assay for detecting low-level fungi in an indoor environment.
The method of bead-beating was identified as the most effective way for spore breakage and fungal DNA release and a fungus-specific PCR assay using only one primer set has been developed for detecting indoor fungi.
Quantitative PCR analysis of selected Aspergillus, Penicillium and Paecilomyces species.
Evidence is provided that the commonly used practices of enumerating Aspergillus and Penicillium as a single group or only by genus can be misleading in understanding the indoor populations of these organisms and their potential health risks.
Indoor fungal contamination: Health risks and measurement methods in hospitals, homes and workplaces
Air, dust and surface sampling strategies are compared, as well as the limits of various methods used to detect and quantify fungal particles and fungal compounds, which identify common needs for future multidisciplinary research and development projects in this field.
Fungal-specific PCR primers developed for analysis of the ITS region of environmental DNA extracts
The complete set of primers was developed with an emphasis on discrimination between plant and fungal sequences and should be particularly useful for studies of fungi where samples also contain high levels of background plant DNA, such as verifying ectomycorrhizal morphotypes or characterizing phylosphere communities.
Seasonal distribution of Alternaria, Aspergillus, Cladosporium and Penicillium species isolated in homes of fungal allergic patients.
The quantitative analysis of the four taxa related with respiratory allergies demonstrated considerable seasonal variability and the greater level of exposure to the four studied taxas occurred inside homes in summer and autumn.