Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study

@article{Libert2017DiscriminationOT,
  title={Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study},
  author={Xavier Libert and Ann Packeu and Fabrice Bureau and Nancy H. C. Roosens and Sigrid C. J. De Keersmaecker},
  journal={BMC Microbiology},
  year={2017},
  volume={17}
}
BackgroundIndoor air pollution caused by fungal contamination is suspected to have a public health impact. Monitoring of the composition of the indoor airborne fungal contaminants is therefore important. To avoid problems linked to culture-dependent protocols, molecular methods are increasingly being proposed as an alternative. Among these molecular methods, the polymerase chain reaction (PCR) and the real-time PCR are the most frequently used tools for indoor fungal detection. However, even if… 
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8 Citations

8 Citations

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TLDR
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Development of high-resolution melting PCR (HRM-PCR) assay to identify native fungal species associated with the wheat endosphere
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TLDR
The developed HRM assay can be used to ascertain the identity of Exophiala species, which may differ in clinical significance, with high accuracy, and its application to identify species directly in clinical samples and/or environmental niches may be possible in the future.
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The MHRM assay offers a simple and fast alternative to culture approach for simultaneous detection of five major bacterial lower respiratory tract infection pathogens and can help clinicians initiate prompt and appropriate antimicrobial treatment, towards reducing the morbidity and mortality of severe respiratory infections.
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It has been established that high levels of aflatoxins in feeds can cause alterations in fish growth as well as the development of cancerous tumors in the liver, in addition to enhancing mortality.

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