Discrimination of three cyst-forming nematodes of the genus Globodera (Nematode: Heteroderidae) from Japan based on PCR-RFLP of ribosomal DNA

@article{Uehara2005DiscriminationOT,
  title={Discrimination of three cyst-forming nematodes of the genus Globodera (Nematode: Heteroderidae) from Japan based on PCR-RFLP of ribosomal DNA},
  author={T. Uehara and A. Kushida and Kenjirou Itou and T. Narabu and Y. Momota},
  journal={Applied Entomology and Zoology},
  year={2005},
  volume={40},
  pages={537-543}
}
The 1TS1-5.8S-1TS2 region of rDNA sequences from three Globodera species found in Japan, G. rostochiensis, G. tabacum and G. hypolysi, were sequenced and compared. Sequence lengths for G. rostochiensis, G. tabacum and G.hypolysi were 1,001, 1,003 and 1,016 bp, respectively. Sequence divergence within the three Globodera species ranged from 3.8 to 14.7%. Eight restriction enzymes (AfaI, AluI, FspI, Hhal, Hinfl, Mval, PmaCI and Sacll) were selected on the basis of sequence information. These… Expand

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