Discrimination of the ITS1 types of Fasciola spp. based on a PCR–RFLP method

  title={Discrimination of the ITS1 types of Fasciola spp. based on a PCR–RFLP method},
  author={Madoka Ichikawa and Tadashi Itagaki},
  journal={Parasitology Research},
Molecular characterization is important for discriminating Fasciola specimens having the deoxyribonucleic acid (DNA) sequences of Fasciola hepatica, Fasciola gigantica, and both Fasciola species, since three Fasciola forms coexist in Asian countries. We have developed a restriction fragment length polymorphism of amplified DNA (PCR–RFLP) of the nuclear ribosomal internal transcribed spacer 1 (ITS1) region in Fasciola species. The band patterns of the fragments digested with a restriction enzyme… CONTINUE READING


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