Discrimination of human HLA-DRB1 alleles by PCR-SSCP (single-strand conformation polymorphism) method.

  title={Discrimination of human HLA-DRB1 alleles by PCR-SSCP (single-strand conformation polymorphism) method.},
  author={Makoto Bannai and Katsushi Tokunaga and Li Lin and Shoji Kuwata and Toshio Mazda and Ichita Amaki and Kiyoshi Fujisawa and Takeo Juji},
  journal={European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics},
  volume={21 1},
A single-strand conformation polymorphism (PCR-SSCP) method has been adopted for discrimination of human HLA-DRB1 alleles. This method enabled the detection of DNA polymorphisms including point mutations at a variety of positions in the DNA fragments of the HLA-DRB1 gene. A total of 27 HLA-DRB1 alleles from 172 healthy donors were analysed using a combination of PCR-SSCP with group-specific amplifications. Application of a small amount of amplified and denatured DNA to non-denaturing… CONTINUE READING
34 Citations
0 References
Similar Papers


Publications citing this paper.
Showing 1-10 of 34 extracted citations

Similar Papers

Loading similar papers…