Discrimination of granulocyte subtypes from light scattering: theoretical analysis using a granulated sphere model.

@article{Yurkin2007DiscriminationOG,
  title={Discrimination of granulocyte subtypes from light scattering: theoretical analysis using a granulated sphere model.},
  author={Maxim A. Yurkin and Konstantin A. Semyanov and Valeri P. Maltsev and Alfons G. Hoekstra},
  journal={Optics express},
  year={2007},
  volume={15 25},
  pages={
          16561-80
        }
}
We perform extensive simulations of light scattering by a granulated sphere in the size and refractive index range of human granulated leucocytes using the discrete dipole approximation. We calculate total and depolarized side scattering signals as a function of the size and refractive indices of cell and granules, and the granule volume fraction. Using typical parameters derived from the literature data on granulocyte morphology, we show that differences between experimentally measured signals… CONTINUE READING

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