Discrimination of damaged/dead cells by propidium iodide uptake in immunofluorescently labeled populations analyzed by phase-sensitive flow cytometry.

@article{Steinkamp1999DiscriminationOD,
  title={Discrimination of damaged/dead cells by propidium iodide uptake in immunofluorescently labeled populations analyzed by phase-sensitive flow cytometry.},
  author={John A. Steinkamp and Bruce E. Lehnert and N M Lehnert},
  journal={Journal of immunological methods},
  year={1999},
  volume={226 1-2},
  pages={
          59-70
        }
}
We report a flow cytometric fluorescence lifetime-based method to discriminate damaged/dead from viable cells in immunofluorescently labeled populations using propidium iodide as a dye-exclusion viability probe. Fluorescence signals from propidium iodide and the anti-thymus cell-surface immunofluorescence marker fluorochromes, phycoerythrin and phycoerythrin/Texas Red (tandem conjugate), which have overlapping emission spectra with propidium iodide, are resolved based on differences in their… CONTINUE READING
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