Discrimination between changes in glucocorticoid receptor expression and activation in rat brain using western blot analysis

@article{Spencer2000DiscriminationBC,
  title={Discrimination between changes in glucocorticoid receptor expression and activation in rat brain using western blot analysis},
  author={Robert L Spencer and Brian A. Kalman and Crystal S Cotter and Terrence Deak},
  journal={Brain Research},
  year={2000},
  volume={868},
  pages={275-286}
}
These studies investigated autoregulation of glucocorticoid receptor (GR) protein expression and activation in rat brain using western blot methodology. By comparing GR immunoblot reactivity present in various tissue subcellular preparations we were able to discriminate between corticosterone-induced changes in GR activation or GR protein expression. Our cytosolic tissue preparation yielded a similar pattern of treatment effects on relative GR as measured by receptor binding assay or western… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-10 OF 52 CITATIONS, ESTIMATED 45% COVERAGE

Differential Regulation of CuZnSOD Expression in Rat Brain by Acute and/or Chronic Stress

  • Cellular and Molecular Neurobiology
  • 2009
VIEW 1 EXCERPT
CITES METHODS
HIGHLY INFLUENCED

FILTER CITATIONS BY YEAR

2001
2018

CITATION STATISTICS

  • 7 Highly Influenced Citations

References

Publications referenced by this paper.
SHOWING 1-10 OF 31 REFERENCES

Luttge, Activated type II receptors in brain cannot rebind glucocorticoids: relationship to progesterone’s antiglucocorticoid actions

W.G.Y.C. Chou
  • Brain Res
  • 1988
VIEW 3 EXCERPTS
HIGHLY INFLUENTIAL

Brain colonic adenocarcinoma cell line , J . Steroid Biochem . Molec . Biol . corticosteroid receptor balance in health and disease

E. Vredugdenhil E. R. de Kloet, M. S. Oitzl, M. Joels
  • Endocr . Rev .
  • 1997

Cidlowski, Ligand-dependent downregulation of stably transfected human glucocorticoid receptors is associated with the loss of functional glucocorticoid

D. L. Bellingham, J.A.M. Sar
  • responsiveness, Mol. Endocrinol
  • 1992
VIEW 3 EXCERPTS

Evidence for glucocorticoid receptor binding and mRNA levels in the rat brain mineralocorticoid receptor facilitation of glucocorticoid receptor - and pituitary

N. Barden Peiffer, M. J. Meaney, P. J. Kim Spencer, B. A. Kalman, M. A. Cole
  • Neurobiol . Aging
  • 1991