Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene

@article{pitalsk2004DiscriminationBT,
  title={Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene},
  author={Eva {\vS}pitalsk{\'a} and Alessandra Torina and Vincenza Cannella and Santo Caracappa and Olivier A. E. Sparagano},
  journal={Parasitology Research},
  year={2004},
  volume={94},
  pages={318-320}
}
Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. lestoquardi only and to discriminate the two species in both tick and blood samples. 

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