Discovery of two novel radical S-adenosylmethionine proteins required for the assembly of an active [Fe] hydrogenase.

@article{Posewitz2004DiscoveryOT,
  title={Discovery of two novel radical S-adenosylmethionine proteins required for the assembly of an active [Fe] hydrogenase.},
  author={Matthew C. Posewitz and Paul W King and Sharon L. Smolinski and Liping Zhang and Michael C. Seibert and M. Ghirardi},
  journal={The Journal of biological chemistry},
  year={2004},
  volume={279 24},
  pages={25711-20}
}
To identify genes necessary for the photoproduction of H(2) in Chlamydomonas reinhardtii, random insertional mutants were screened for clones unable to produce H(2). One of the identified mutants, denoted hydEF-1, is incapable of assembling an active [Fe] hydrogenase. Although the hydEF-1 mutant transcribes both hydrogenase genes and accumulates full-length hydrogenase protein, H(2) production activity is not observed. The HydEF protein contains two unique domains that are homologous to two… CONTINUE READING
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