Discovery of nuclear and plastid microsatellites, and other key genomic information, in the rare endemic plant (Caulanthus amplexicaulis var. barbarae) using minimal 454 pyrosequencing

  title={Discovery of nuclear and plastid microsatellites, and other key genomic information, in the rare endemic plant (Caulanthus amplexicaulis var. barbarae) using minimal 454 pyrosequencing},
  author={A. Burrell and E. No and A. Pepper},
  journal={Conservation Genetics Resources},
Next-generation Roche 454 pyrosequencing was used to rapidly identify 149 novel nuclear microsatellite loci and five plastid microsatellites from non-enriched genomic DNA of the rare endemic Santa Barbara Jewelflower (Caulanthus amplexicaulis var. barbarae). Analysis of this dataset showed a high level of orthology to annotated genes of the model plant Arabidopsis thaliana, as well as low levels of repetitive DNA sequences (particularly transposon-like elements). Thus, a modest sequencing… Expand

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