Discovery of human antibodies to cell surface antigens by capture lift screening of phage-expressed antibody libraries.

@article{Watkins1998DiscoveryOH,
  title={Discovery of human antibodies to cell surface antigens by capture lift screening of phage-expressed antibody libraries.},
  author={Jeffry D. Watkins and Greg Beuerlein and Herren Wu and P Mcfadden and James D. Pancook and William D. Huse},
  journal={Analytical biochemistry},
  year={1998},
  volume={256 2},
  pages={169-77}
}
An assay for the rapid identification and cloning of antibody fragments (Fabs) reactive with cell surface antigens was established and used to identify Fabs selectively reactive with tumor cell surface antigens. The Fabs were produced by a phage expression system and screened by a modified plaque lift approach in which nitrocellulose filters were coated with an anti-immunoglobulin reagent and blocked with bovine serum albumin prior to application to the phage-infected bacterial lawn… CONTINUE READING