Discovery of a Cytokine and Its Receptor by Functional Screening of the Extracellular Proteome

  title={Discovery of a Cytokine and Its Receptor by Functional Screening of the Extracellular Proteome},
  author={Haishan Lin and Ernestine Lee and Kevin Hestir and Cindy Leo and Minmei Huang and Elizabeth Bosch and Robert F. Halenbeck and Ge Wu and Aileen J Zhou and Dirk Behrens and Diane L Hollenbaugh and Thomas Linnemann and Minmin Qin and Justin Jong-Leong Wong and Keting Emeryville Chu and Stephen. Doberstein and Lewis T. Williams},
  pages={807 - 811}
To understand the system of secreted proteins and receptors involved in cell-cell signaling, we produced a comprehensive set of recombinant secreted proteins and the extracellular domains of transmembrane proteins, which constitute most of the protein components of the extracellular space. Each protein was tested in a suite of assays that measured metabolic, growth, or transcriptional responses in diverse cell types. The pattern of responses across assays was analyzed for the degree of… 
Deorphanization of the human leukocyte tyrosine kinase (LTK) receptor by a signaling screen of the extracellular proteome
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The Secreted Form of Transmembrane Protein 98 Promotes the Differentiation of T Helper 1 Cells.
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  • Biology
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  • 2015
Function analysis showed that eukaryotic recombinant TMEM98 (rTMEM98) promoted the differentiation of Th1 cells under both antigen-nonspecific and antigen-specific Th1-skewing conditions, which will be helpful in further understanding the development of Th 1 cells.
Screening the mammalian extracellular proteome for regulators of embryonic human stem cell pluripotency
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Live Cell Membranome cDNA Screen: A Novel Homogenous Live Cell Binding Assay to Study Membrane Protein-Ligand Interaction
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Quantitative Proteomics for Investigation of Secreted Factors: Focus on Muscle Secretome
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A Pilot Screen of a Novel Peptide Hormone Library Identified Candidate GPR83 Ligands
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Secretome-Based Screening in Target Discovery
The secretome library offers several advantages compared with other libraries used for target discovery, which make it ideal for testing in physiologically relevant cell types, and therefore it represents an attractive approach to phenotypic target discovery.


Colony-stimulating factor-1 in immunity and inflammation.
Inhibition of colony-stimulating-factor-1 signaling in vivo with the orally bioavailable cFMS kinase inhibitor GW2580.
The ability of GW2580 to chronically inhibit CSF-1 signaling through cFMS kinase in normal and tumor cells in vivo makesGW2580 a useful tool in assessing the role of cF MS kinaseIn normal and disease processes.
The biology of interleukin-2 and interleukin-15: implications for cancer therapy and vaccine design
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  • 2006
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Transgenic mice expressing human fibroblast growth factor-19 display increased metabolic rate and decreased adiposity.
It is reported that FGF19 transgenic mice had a significant and specific reduction in fat mass that resulted from an increase in energy expenditure and did not become obese or diabetic on a high fat diet.
FGF-21 as a novel metabolic regulator.
It is concluded that FGF-21, which was discovered to be a potent regulator of glucose uptake in mouse 3T3-L1 and primary human adipocytes, exhibits the therapeutic characteristics necessary for an effective treatment of diabetes.
FGF-19, a novel fibroblast growth factor with unique specificity for FGFR4.
FGF-19 is a high affinity, heparin dependent ligand for FGFR4 and is the first member of the FGF family to show exclusive binding toFGFR4.
Targeted disruption of the mouse colony-stimulating factor 1 receptor gene results in osteopetrosis, mononuclear phagocyte deficiency, increased primitive progenitor cell frequencies, and reproductive defects.
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Machine learning
Machine learning addresses many of the same research questions as the fields of statistics, data mining, and psychology, but with differences of emphasis.