Discovery of a Bacterial Glycoside Hydrolase Family 3 (GH3) β-Glucosidase with Myrosinase Activity from a Citrobacter Strain Isolated from Soil.

@article{Albaser2016DiscoveryOA,
  title={Discovery of a Bacterial Glycoside Hydrolase Family 3 (GH3) β-Glucosidase with Myrosinase Activity from a Citrobacter Strain Isolated from Soil.},
  author={Abdulhadi Ali Albaser and Eleanna Kazana and Mark H. Bennett and Fatma Cebeci and Vijitra Luang-In and Pietro D. Spanu and John Trevor Rossiter},
  journal={Journal of agricultural and food chemistry},
  year={2016},
  volume={64 7},
  pages={1520-7}
}
A Citrobacter strain (WYE1) was isolated from a UK soil by enrichment using the glucosinolate sinigrin as sole carbon source. The enzyme myrosinase was purified using a combination of ion exchange and gel filtration to give a pure protein of approximately 66 kDa. The N-terminal amino acid and internal peptide sequence of the purified protein were determined and used to identify the gene, which, based on InterPro sequence analysis, belongs to the family GH3, contains a signal peptide, and is a… CONTINUE READING

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