Discovery of Protein Modifications Using Differential Tandem Mass Spectrometry Proteomics.

  title={Discovery of Protein Modifications Using Differential Tandem Mass Spectrometry Proteomics.},
  author={Paolo Cifani and Zhi Li and Dan-ni Luo and Mark Grivainis and Andrew M. Intlekofer and David Feny{\"o} and Alex Kentsis},
  journal={Journal of proteome research},
Recent studies have revealed diverse amino acid, post-translational, and noncanonical modifications of proteins in diverse organisms and tissues. However, their unbiased detection and analysis remain hindered by technical limitations. Here, we present a spectral alignment method for the identification of protein modifications using high-resolution mass spectrometry proteomics. Termed SAMPEI for spectral alignment-based modified peptide identification, this open-source algorithm is designed for… 

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