Discovery and identification of new D13S317 primer binding site mutations.

@article{Grgicak2006DiscoveryAI,
  title={Discovery and identification of new D13S317 primer binding site mutations.},
  author={Catherine M. Grgicak and Sue F. Rogers and Carl Mauterer},
  journal={Forensic science international},
  year={2006},
  volume={157 1},
  pages={
          36-9
        }
}
During the course of conventional testing of CODIS standards at the Alabama Department of Forensic Sciences, a sample with a heterozygous null genotype at D13S317 was discovered using the PowerPlex 1.1 kit (Promega, Madison, WI). The loss of both alleles was confirmed when the sample was amplified using PowerPlex 1.2 primers and resulted in a 9, 10 genotype at this locus. To determine the cause of the silent alleles, the ADFS designed D13S317 primers which encompassed the PowerPlex 1.1 D13S317… CONTINUE READING