Discovery and characterization of new transcripts from RNA-seq data in mouse CD4(+) T cells.

@article{Hutchins2012DiscoveryAC,
  title={Discovery and characterization of new transcripts from RNA-seq data in mouse CD4(+) T cells.},
  author={Andrew P Hutchins and St{\'e}phane Poulain and Hodaka Fujii and Diego Miranda-Saavedra},
  journal={Genomics},
  year={2012},
  volume={100 5},
  pages={303-13}
}
Despite the routine application of RNA-seq technology to profile cellular transcriptomes and report novel splice variants, the identification and validation of new transcripts remain underexplored. We prepared two RNA-seq libraries from resting and T cell receptor-stimulated mouse CD4(+) T cells. Transcripts unknown to Ensembl represent as much as 5% of the assembled transcripts and are robustly expressed but do not show the same degree of evolutionary conservation or exon distribution of known… CONTINUE READING

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