Discovery and characterization of a peptide motif that specifically recognizes a non-native conformation of human IgG induced by acidic pH conditions.

@article{Sakamoto2009DiscoveryAC,
  title={Discovery and characterization of a peptide motif that specifically recognizes a non-native conformation of human IgG induced by acidic pH conditions.},
  author={Kotaro Sakamoto and Yuji Ito and Takaaki Hatanaka and Preeti Soni and Toshiyuki Mori and Kazuhisa Sugimura},
  journal={The Journal of biological chemistry},
  year={2009},
  volume={284 15},
  pages={9986-93}
}
In therapeutic antibody preparation, acidic pH conditions are generally used for elution from Protein A affinity column of IgG or for its viral inactivation. Exposing IgG to low pH conditions induces conformational changes, leading to its functional damage or loss, although the mechanisms have not been fully elucidated. In this study using random peptide T7 phage display libraries, we isolated a unique and novel peptide motif that specifically recognized the non-native conformer (acid conformer… CONTINUE READING

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BIOLOGICAL CHEMISTRY 9993 by gest on Feruary 3, 2015 hp://w w w .jb.org/ D

  • M. J. Thies, R. Kammermeier, K. Richter, J. Buchner
  • J. Mol. Biol
  • 2001

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