Discovery and characterization of Sip1A: a novel secreted protein from Bacillus thuringiensis with activity against coleopteran larvae

@article{Donovan2006DiscoveryAC,
  title={Discovery and characterization of Sip1A: a novel secreted protein from Bacillus thuringiensis with activity against coleopteran larvae},
  author={William P. Donovan and James T. Engleman and Judith C. Donovan and James A. Baum and Greg J. Bunkers and David J. Chi and William Clinton and Leigh English and Gregory R. Heck and Oliver M. Ilagan and Karina C. Krasomil-Osterfeld and John W. Pitkin and James K. Roberts and Matthew R. Walters},
  journal={Applied Microbiology and Biotechnology},
  year={2006},
  volume={72},
  pages={713-719}
}
Bioassay screening of Bacillus thuringiensis culture supernatants identified strain EG2158 as having larvicidal activity against Colorado potato beetle (Leptinotarsa decemlineata) larvae. Ion-exchange fractionation of the EG2158 culture supernatant resulted in the identification of a protein designated Sip1A (secreted insecticidal protein) of approximately 38 kDa having activity against Colorado potato beetle (CPB). An oligonucleotide probe based on the N-terminal sequence of the purified Sip1A… CONTINUE READING

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