Discovery and assay of single-nucleotide polymorphisms in barley (Hordeum vulgare)

@article{Kanazin2002DiscoveryAA,
  title={Discovery and assay of single-nucleotide polymorphisms in barley (Hordeum vulgare)},
  author={Vladimir Kanazin and Hope Talbert and Deven See and Phil DeCamp and Eviatar Nevo and Tom Blake},
  journal={Plant Molecular Biology},
  year={2002},
  volume={48},
  pages={529-537}
}
The least ambiguous genetic markers are those based on completely characterized DNA sequence polymorphisms. Unfortunately, assaying allele states by allele sequencing is slow and cumbersome. The most desirable type of genetic marker would be unambiguous, inexpensive to assay and would be assayable singly or in parallel with hundreds of other markers (multiplexable). In this report we sequenced alleles at 54 barley (Hordeum vulgare ssp. vulgare) loci, 38 of which contained single-nucleotide… CONTINUE READING
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