Discontinuity and limited linkage in the homologous recombination system of a hyperthermophilic archaeon.

@article{Grogan2010DiscontinuityAL,
  title={Discontinuity and limited linkage in the homologous recombination system of a hyperthermophilic archaeon.},
  author={Dennis W. Grogan and Jananie Rockwood},
  journal={Journal of bacteriology},
  year={2010},
  volume={192 18},
  pages={4660-8}
}
Genetic transformation of Sulfolobus acidocaldarius by a multiply marked pyrE gene provided a high-resolution assay of homologous recombination in a hyperthermophilic archaeon. Analysis of 100 Pyr(+) transformants revealed that this recombination system could transfer each of 23 nonselected base pair substitutions to the recipient chromosome along with the selected marker. In 30% of the recombinants, donor markers were transferred as multiple blocks. In at least 40% of the recombinants, donor… CONTINUE READING