Discoidin-binding polysaccharide from Dictyostelium discoideum.

  title={Discoidin-binding polysaccharide from Dictyostelium discoideum.},
  author={Douglas N. W. Cooper and Simon C. Lee and S H Barondes},
  journal={The Journal of biological chemistry},
  volume={258 14},
Extracts of Dictyostelium discoideum grown axenically in a chemically defined medium were evaluated for binding to discoidin I and discoidin II, endogenous lectins of this slime mold. Binding activity was measured by competitive inhibition of 125I-lactosyl-bovine serum albumin binding to the immobilized lectins. With the solubilization procedure used extracts of vegetative cells and of early aggregates had no significant inhibitory activity, but an abundant discoidin-binding substance was… CONTINUE READING