Disaggregation of Aggregated Platelets by Savignygrin, a αIIbβ3 Antagonist from Ornithodoros Savignyi

@article{Mans2004DisaggregationOA,
  title={Disaggregation of Aggregated Platelets by Savignygrin, a $\alpha$IIb$\beta$3 Antagonist from Ornithodoros Savignyi},
  author={B. Mans and A. Louw and A. Neitz},
  journal={Experimental \& Applied Acarology},
  year={2004},
  volume={27},
  pages={231-239}
}
Ticks control their host's hemostatic system by secretion of bioactive components during feeding that inhibit blood coagulation and platelet aggregation. Dissolution of platelets that have already aggregated can enhance control over the hemostatic system. It has been shown that disaggregation of aggregated platelets by the enzyme apyrase was accompanied by a shape change from the aggregated spherical form back to the discoid form associated with un-activated platelets. The present study… Expand

Figures from this paper

The mechanism of αIIbβ3 antagonism by savignygrin and its implications for the evolution of anti-hemostatic strategies in soft ticks
Abstract Savignygrin, a αIIbβ3 antagonist presents the RGD sequence on the substrate-binding loop of the (BPTI-fold). This study investigated whether this is the only integrin-targeting motifExpand
A novel fibrinolytic metalloproteinase, barnettlysin-I from Bothrops barnetti (Barnett´s pitviper) snake venom with anti-platelet properties.
TLDR
A new P-I class snake venom metalloproteinase (SVMP), barnettlysin-I (Bar-I), isolated from the venom of Bothrops barnetti, is reported and its fibrinolytic and antithrombotic potential is evaluated. Expand
Characterization of anti-hemostatic factors in the argasid, Argas monolakensis: implications for the evolution of blood-feeding in the soft tick family.
TLDR
The Argas derived anti-hemostatic factors bind to mammalian targets with affinities similar to that observed for their orthologs in the Ornithodoros genus, implying that the anti- hemostatics factors evolved in the ancestral soft tick lineage and has been maintained in soft tick species from two distinct genera with different host preferences. Expand
Tick-derived Kunitz-type inhibitors as antihemostatic factors.
TLDR
The unsurpassed versatility of tick-derived Kunitz inhibitors establishes them as valuable tools for biochemical investigations, but also as lead compounds for the development of novel antithrombotics. Expand
Anticoagulants from Hematophagous
TLDR
This chapter will focus on anticoagulant molecules described until now from hematophagous animals, pointing out that they can also display non-hemostatic functions as antitumor, bringing new perspectives for the study of these molecules. Expand
BmTI-A, a Kunitz type inhibitor from Rhipicephalus microplus able to interfere in vessel formation.
Rhipicephalus microplus is an ectoparasite responsible for transmissions of babesiosis and anaplasmosis causing large losses to livestock production. To survive R. microplus tick produces severalExpand
Amblyomma americanum tick saliva serine protease inhibitor 6 is a cross‐class inhibitor of serine proteases and papain‐like cysteine proteases that delays plasma clotting and inhibits platelet aggregation
TLDR
It is demonstrated that AamS6 is a cross‐class inhibitor of both serine‐ and papain‐like cysteine proteases that has apparent antihaemostatic functions and is most likely not involved with the tick's evasion of the host's complement defense mechanism. Expand
The sand tampan, Ornithodoros savignyi , as a model for tick-host interactions : review article
TLDR
The study of anti-haemostatic components secreted by the tampan indicates a close evolutionary relationship between different anti-HAEMostatic mechanisms, which has several implications for general tick biology, the most important being that the main tick families adapted independently to a blood-feeding lifestyle and that the different forms of tick toxicoses have distinct origins. Expand
Insight into the sialome of the castor bean tick, Ixodes ricinus
TLDR
This work contributes to the growing list of proteins associated with tick feeding and sheds more light on the dynamics of the gene expression during tick feeding, and corroborate previous evidence of gene duplication in the evolution of ticks. Expand
Adaptation of ticks to a blood-feeding environment: evolution from a functional perspective.
  • B. Mans, A. Neitz
  • Biology, Medicine
  • Insect biochemistry and molecular biology
  • 2004
TLDR
It is concluded that the main tick families adapted independently to a blood-feeding environment and this has several implications for future tick research in terms of tick genome projects and vaccine development. Expand
...
1
2
...

References

SHOWING 1-10 OF 26 REFERENCES
Disaggregation of aggregated platelets by apyrase from the tick, Ornithodoros savignyi
TLDR
It is shown that apyrase can disaggregate platelets even after secondary aggregation and degranulation of platelets has taken place, one of the main barriers encountered by ticks during feeding and counteraction of this process by ticks is an important factor for successful feeding. Expand
Savignygrin, a Platelet Aggregation Inhibitor from the Soft TickOrnithodoros savignyi, Presents the RGD Integrin Recognition Motif on the Kunitz-BPTI Fold*
TLDR
This is the first report of a platelet aggregation inhibitor that presents the RGD motif using the Kunitz-BPTI protein fold, and indicates the specificity of savignygrin toward αIIbβ3. Expand
Disagregin is a fibrinogen receptor antagonist lacking the Arg-Gly-Asp sequence from the tick, Ornithodoros moubata.
TLDR
Disagregin is unique both in structure and function and may serve as a useful tool for the design of therapeutically useful antithrombotic agents. Expand
Apyrase Activity and Platelet Aggregation Inhibitors in the Tick Ornithodoros Savignyi (Acari: Argasidae)
TLDR
The results suggest the presence of two or more anti-platelet factors present in the salivary glands of this tick species. Expand
Isolation of an inhibitor selective for collagen-stimulated platelet aggregation from the soft tick Ornithodoros moubata.
TLDR
The amino acid sequences of peptides derived from proteolytic cleavage of mouBatin suggest that moubatin is a unique protein, consistent with its novel functional activity. Expand
Savignin, a potent thrombin inhibitor isolated from the salivary glands of the tick Ornithodoros savignyi (Acari: Argasidae).
TLDR
Savignin inhibits thrombin-induced platelet aggregation, but has no effect on ADP- or collagen-induced aggregation, and Plasmin, factor Xa, and trypsin are not inhibited by savignin. Expand
Purification and characterization of apyrase from the tick, Ornithodoros savignyi.
TLDR
The purified enzyme conformed to properties associated with apyrases, including low substrate specificity, a dependence on bivalent metal ions and an insensitivity to normal ATPase inhibitors and sulfhydryl group reagents, indicating that apyrase fulfils an important function during tick feeding. Expand
On the Structure and Function of Platelet Integrin αIIbβ3, the Fibrinogen Receptor
  • J. Calvete
  • Chemistry, Medicine
  • Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
  • 1995
TLDR
The aim of this minireview is to summarize many functional and structural data from different laboratories in the perspective of an emerging model that may help to understand structure-function relationships of GPIIb/IIIa and of other members of the integrin family. Expand
Monoclonal antibodies bound to subunits of the integrin GPIIb-IIIa are internalized and interfere with filopodia formation and platelet aggregation.
TLDR
It is concluded that aggregation requires the formation of long, slender filopodia, probably directed by cytoskeletal rearrangements after activation, and that the transmembrane GPIIb-IIIa complex may play a role in signaling these events. Expand
Variabilin, a Novel RGD-containing Antagonist of Glycoprotein IIb-IIIa and Platelet Aggregation Inhibitor from the Hard Tick Dermacentor variabilis*
TLDR
A novel inhibitor of human platelet aggregation, named variabilin, was isolated from salivary glands of the hard tick Dermacentor variabilis using a combination of gel filtration and high pressure liquid chromatography, and is the first RGD-containing antagonist isolated from ticks. Expand
...
1
2
3
...