Dirigent proteins: molecular characteristics and potential biotechnological applications

@article{Pickel2013DirigentPM,
  title={Dirigent proteins: molecular characteristics and potential biotechnological applications},
  author={Benjamin Pickel and Andreas Schaller},
  journal={Applied Microbiology and Biotechnology},
  year={2013},
  volume={97},
  pages={8427-8438}
}
Dirigent proteins (DIRs) are thought to play important roles in plant secondary metabolism. They lack catalytic activity but direct the outcome of bimolecular coupling reactions toward regio- and stereospecific product formation. Functionally described DIRs confer specificity to the oxidative coupling of coniferyl alcohol resulting in the preferred production of either (+)- or (−)-pinoresinol, which are the first intermediates in the enantiocomplementary pathways for lignan biosynthesis. DIRs… 

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References

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A model of dirigent proteins derived from structural and functional similarities with allene oxide cyclase and lipocalins

It is shown here that the dirigent protein AtDIR6 from Arabidopsis’thaliana is a homodimeric all‐beta protein in the superfamily of calycins, suggesting that dirigent proteins evolved from hydrophobic ligand‐binding proteins.

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Lignans are a widely distributed class of natural products, whose functions and distribution suggest that they are one of the earliest forms of defense to have evolved in vascular plants; some, such

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Analytical ultracentrifugation, sedimentation velocity, and sedimentation equilibrium analyses of the native dirigent protein in open solution confirmed further its dimeric nature as well as a propensity to aggregate, with the latter being dependent upon both temperature and solution ionic strength.

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