Directionality of replication fork movement determined by two-dimensional native-native DNA agarose gel electrophoresis.


The analysis of replication intermediates by the neutral-neutral two-dimensional agarose gel technique allows determining the chromosomal positions where DNA replication initiates, whether replication forks pause or stall at specific sites, or whether two DNA molecules undergo DNA recombination events. This technique does not, however, immediately tell in… (More)
DOI: 10.1007/978-1-62703-565-1_5


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