Directionality of individual kinesin-5 Cin8 motors is modulated by loop 8, ionic strength and microtubule geometry.

@article{GersonGurwitz2011DirectionalityOI,
  title={Directionality of individual kinesin-5 Cin8 motors is modulated by loop 8, ionic strength and microtubule geometry.},
  author={Adina Gerson-Gurwitz and Christina Thiede and Natalia Movshovich and Vladimir Fridman and Maria Podolskaya and Tsafi Danieli and Stefan Lak{\"a}mper and Dieter Robert Klopfenstein and Christoph F Schmidt and Larisa Gheber},
  journal={The EMBO journal},
  year={2011},
  volume={30 24},
  pages={4942-54}
}
Kinesin-5 motors fulfil essential roles in mitotic spindle morphogenesis and dynamics as slow, processive microtubule (MT) plus-end directed motors. The Saccharomyces cerevisiae kinesin-5 Cin8 was found, surprisingly, to switch directionality. Here, we have examined directionality using single-molecule fluorescence motility assays and live-cell microscopy. On spindles, Cin8 motors mostly moved slowly (∼25 nm/s) towards the midzone, but occasionally also faster (∼55 nm/s) towards the spindle… CONTINUE READING

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