Directional differentiation of chicken primordial germ cells into adipocytes, neuron-like cells, and osteoblasts.

@article{Li2010DirectionalDO,
  title={Directional differentiation of chicken primordial germ cells into adipocytes, neuron-like cells, and osteoblasts.},
  author={B. C. Li and Ziqiang Tian and Meng Sun and Q Xu and Xu Wang and Yan Ru Qin and Fuhua Xu and B. G. Gao and Klein Heuy-Ching Wang and Hui Chuan Sun and Gina H Chen},
  journal={Molecular reproduction and development},
  year={2010},
  volume={77 9},
  pages={795-801}
}
Primordial germ cells (PGCs) are useful for producing transgenic chickens and preserving genetic material in avian species. In this study, we investigated the in vitro differentiation potential of chicken PGCs into different cell types. For differentiation into adipocytes, chicken PGCs were cultured for 21 days in induction media containing dexamethasone, insulin and/or 3-isobutyl-1-methylxanthine (IBMX), and differentiation rates ranging from 74% to 91% were identified by oil red-O and… CONTINUE READING