Directional cloning of native PCR products with preformed sticky ends (Autosticky PCR)

  title={Directional cloning of native PCR products with preformed sticky ends (Autosticky PCR)},
  author={J{\'o}zsef G{\'a}l and Roland Schnell and S{\'a}ndor Szekeres and Mikl{\'o}s K{\'a}lm{\'a}n},
  journal={Molecular and General Genetics MGG},
A novel method for the directional cloning of native PCR products was developed. Abasic sites in DNA templates make DNA polymerases stall at the site during synthesis of the complementary strand. Since the 5′ ends of PCR product strands contain built-in amplification primers, abasic sites within the primers result in the formation of 5′ single-stranded overhangs at the ends of the PCR product, enabling its direct ligation to a suitably cleaved cloning vector without any further modification… CONTINUE READING

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