Directing phage selections towards specific epitopes.

  title={Directing phage selections towards specific epitopes.},
  author={H. L. Parsons and J. Earnshaw and J. Wilton and K. S. Johnson and P. Schueler and W. Mahoney and J. McCafferty},
  journal={Protein engineering},
  volume={9 11},
It is possible to direct selections from antibody repertoires displayed on filamentous phage towards unique epitopes on protein antigens by competing with related molecules. A phage display repertoire of human single chain Fvs (scFvs) was panned three times against foetal haemoglobin (HbF). The selection was dominated by one clone with a Kd of 10 nM but yielded at least 17 others, all of which bound HbF but crossreacted with adult haemoglobin (HbA). To direct selection towards HbF-specific… Expand
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