Directed natural product biosynthesis gene cluster capture and expression in the model bacterium Bacillus subtilis

@inproceedings{Li2015DirectedNP,
  title={Directed natural product biosynthesis gene cluster capture and expression in the model bacterium Bacillus subtilis},
  author={Yongxin Li and Zhongrui Li and Kazuya Yamanaka and Ying Xu and Weipeng Zhang and Hera Vlamakis and Roberto Kolter and Bradley S Moore and Pei-Yuan Qian},
  booktitle={Scientific reports},
  year={2015}
}
Bacilli are ubiquitous low G+C environmental Gram-positive bacteria that produce a wide assortment of specialized small molecules. Although their natural product biosynthetic potential is high, robust molecular tools to support the heterologous expression of large biosynthetic gene clusters in Bacillus hosts are rare. Herein we adapt transformation-associated recombination (TAR) in yeast to design a single genomic capture and expression vector for antibiotic production in Bacillus subtilis… CONTINUE READING
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