Directed excision of a transgene from the plant genome

Sandra H. Russell, Joyce L. Hoopes, Joan T. Odell
Published 1992 in Molecular and General Genetics MGG

Abstract

The effectiveness of loxP-Cre directed excision of a transgene was examined using phenotypic and molecular analyses. Two methods of combining the elements of this system, re-transformation and cross pollination, were found to produce different degrees of excision in the resulting plants. Two linked traits, β-glucuronidase (GUS) and a gene encoding… (More)

DOI: 10.1007/BF00272344

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References

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Site-directed recombination in the genome of transgenic tobacco

Joan Odell, Perry Caimi, Brian Sauer, Sandra Russell
Molecular and General Genetics MGG
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Relative strengths of the 35S califlower mosaic virus, 1′, 2′, and nopaline synthase promoters in transformed tobacco sugarbeet and oilseed rape callus tissue

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Molecular and General Genetics MGG
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Detection and abundance of mRNA and protein encoded by transposable element Activator (Ac) in maize

Heidi Fußwinkel, Sylvia Schein, Ulrike Courage, Peter Starlinger, Reinhard Kunze
Molecular and General Genetics MGG
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Differential inactivation and methylation of a transgene in plants by two suppressor loci containing homologous sequences

M. A. Matzke, A. J. M. Matzke
Plant Molecular Biology
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Gene transfer with subsequent removal of the selection gene from the host genome.

E C Dale, D W Ow
Proceedings of the National Academy of Sciences…
1991

Transformation of a partial nopaline synthase gene into tobacco suppresses the expression of a resident wild-type gene.

D R Goring, L Thomson, S J Rothstein
Proceedings of the National Academy of Sciences…
1991

Flavonoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression.

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The Plant cell
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Intra- and intermolecular site-specific recombination in plant cells mediated by bacteriophage P1 recombinase.

E C Dale, D W Ow
Gene
1990

Directed excision of a transgene from the plant genome

Abstract

Topics

7 Figures and Tables

Cited By

Elimination of Transgenic Sequences in Plants by Cre Gene Expression

Generation of a homozygous fertilization-defective gcs1 mutant by heat-inducible removal of a rescue gene

Salicylic-Acid-Induced Self-excision of the Marker Gene nptII from Transgenic Tomato Using the Cre–loxP System

Evaluation of CRE-mediated excision approaches in Arabidopsis thaliana

Generating Marker-Free Transgenic Wheat Using Minimal Gene Cassette and Cold-Inducible Cre/Lox System

Cre-mediated seed-specific transgene excision in tobacco

A Cre/loxP-mediated self-activating gene excision system to produce marker gene free transgenic soybean plants

FLP-mediated site-specific recombination for genome modification in turfgrass

Site-specific recombination in Zea mays

Cre recombinase expression can result in phenotypic aberrations in plants

References

Binary vectors. In: Gelvin SB, Schilperoort RA (eds) Plant molecular biology manual

Site-directed recombination in the genome of transgenic tobacco

Relative strengths of the 35S califlower mosaic virus, 1′, 2′, and nopaline synthase promoters in transformed tobacco sugarbeet and oilseed rape callus tissue

Molecular biology of plants

Detection and abundance of mRNA and protein encoded by transposable element Activator (Ac) in maize

Differential inactivation and methylation of a transgene in plants by two suppressor loci containing homologous sequences

Gene transfer with subsequent removal of the selection gene from the host genome.

Transformation of a partial nopaline synthase gene into tobacco suppresses the expression of a resident wild-type gene.

Flavonoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression.

Intra- and intermolecular site-specific recombination in plant cells mediated by bacteriophage P1 recombinase.

Slides referencing similar topics

Directed excision of a transgene from the plant genome

Abstract

Topics

7 Figures and Tables

Cited By

Elimination of Transgenic Sequences in Plants by Cre Gene Expression

Generation of a homozygous fertilization-defective gcs1 mutant by heat-inducible removal of a rescue gene

Salicylic-Acid-Induced Self-excision of the Marker Gene nptII from Transgenic Tomato Using the Cre–loxP System

Evaluation of CRE-mediated excision approaches in Arabidopsis thaliana

Generating Marker-Free Transgenic Wheat Using Minimal Gene Cassette and Cold-Inducible Cre/Lox System

Cre-mediated seed-specific transgene excision in tobacco

A Cre/loxP-mediated self-activating gene excision system to produce marker gene free transgenic soybean plants

FLP-mediated site-specific recombination for genome modification in turfgrass

Site-specific recombination in Zea mays

Cre recombinase expression can result in phenotypic aberrations in plants

References

Binary vectors. In: Gelvin SB, Schilperoort RA (eds) Plant molecular biology manual

Site-directed recombination in the genome of transgenic tobacco

Relative strengths of the 35S califlower mosaic virus, 1′, 2′, and nopaline synthase promoters in transformed tobacco sugarbeet and oilseed rape callus tissue

Molecular biology of plants

Detection and abundance of mRNA and protein encoded by transposable element Activator (Ac) in maize

Differential inactivation and methylation of a transgene in plants by two suppressor loci containing homologous sequences

Gene transfer with subsequent removal of the selection gene from the host genome.

Transformation of a partial nopaline synthase gene into tobacco suppresses the expression of a resident wild-type gene.

Flavonoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression.

Intra- and intermolecular site-specific recombination in plant cells mediated by bacteriophage P1 recombinase.

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Slides referencing similar topics