Directed evolution of polypropylene and polystyrene binding peptides

@article{Rbsam2018DirectedEO,
  title={Directed evolution of polypropylene and polystyrene binding peptides},
  author={Kristin R{\"u}bsam and L. Weber and Felix Jakob and U. Schwaneberg},
  journal={Biotechnology and Bioengineering},
  year={2018},
  volume={115},
  pages={321 - 330}
}
  • Kristin Rübsam, L. Weber, +1 author U. Schwaneberg
  • Published 2018
  • Chemistry, Medicine
  • Biotechnology and Bioengineering
  • Surface functionalization of biological inert polymers (e.g., polypropylene PP; polystyrene PS) with material binding peptides facilitates an efficient immobilization of enzymes, bioactive peptides or antigens at ambient temperature in water. The developed robust directed evolution protocol enables to tailor polymer binding anchor peptides (PBPs) for efficient binding under application conditions. Key for a successful directed evolution campaign was to develop an epPCR protocol with a very high… CONTINUE READING
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