Directed evolution of novel polymerase activities: mutation of a DNA polymerase into an efficient RNA polymerase.

@article{Xia2002DirectedEO,
  title={Directed evolution of novel polymerase activities: mutation of a DNA polymerase into an efficient RNA polymerase.},
  author={Gang Xia and Liangjing Chen and Takashi Sera and Ming Fa and Peter G. Schultz and Floyd E Romesberg},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2002},
  volume={99 10},
  pages={6597-602}
}
The creation of novel enzymatic function is of great interest, but remains a challenge because of the large sequence space of proteins. We have developed an activity-based selection method to evolve DNA polymerases with RNA polymerase activity. The Stoffel fragment (SF) of Thermus aquaticus DNA polymerase I is displayed on a filamentous phage by fusing it to a pIII coat protein, and the substrate DNA template/primer duplexes are attached to other adjacent pIII coat proteins. Phage particles… CONTINUE READING

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Phage Display of Peptides and Proteins: A Laboratory Manual (Academic, San Diego)

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