Directed evolution of ligand dependence: small-molecule-activated protein splicing.

  title={Directed evolution of ligand dependence: small-molecule-activated protein splicing.},
  author={Allen R. Buskirk and Y P Ong and Zev J Gartner and David R Liu},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={101 29},
Artificial molecular switches that modulate protein activities in response to synthetic small molecules would serve as tools for exerting temporal and dose-dependent control over protein function. Self-splicing protein elements (inteins) are attractive starting points for the creation of such switches, because their insertion into a protein blocks the target protein's function until splicing occurs. Natural inteins, however, are not known to be regulated by small molecules. We evolved an intein… CONTINUE READING
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