Directed evolution of a recombinase that excises the provirus of most HIV-1 primary isolates with high specificity

@article{Karpinski2016DirectedEO,
  title={Directed evolution of a recombinase that excises the provirus of most HIV-1 primary isolates with high specificity},
  author={Janet Karpinski and I. Forftrat Hauber and Jan Chemnitz and Carola Sch{\"a}fer and Maciej Paszkowski-Rogacz and Deboyoti Chakraborty and Niklas Beschorner and Helga Hofmann-Sieber and Ulrike C. Lange and Adam Grundhoff and Karl Hackmann and Evelin Schrock and Josephine Abi-Ghanem and M. Teresa Pisabarro and Vineeth Surendranath and Axel Schambach and Christoph Lindner and Jan van Lunzen and Joachim Hauber and Frank Buchholz},
  journal={Nature Biotechnology},
  year={2016},
  volume={34},
  pages={401-409}
}
Current combination antiretroviral therapies (cART) efficiently suppress HIV-1 reproduction in humans, but the virus persists as integrated proviral reservoirs in small numbers of cells. To generate an antiviral agent capable of eradicating the provirus from infected cells, we employed 145 cycles of substrate-linked directed evolution to evolve a recombinase (Brec1) that site-specifically recognizes a 34-bp sequence present in the long terminal repeats (LTRs) of the majority of the clinically… CONTINUE READING
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