Directed evolution of a novel N-carbamylase/D-hydantoinase fusion enzyme for functional expression with enhanced stability.

@article{Kim2000DirectedEO,
  title={Directed evolution of a novel N-carbamylase/D-hydantoinase fusion enzyme for functional expression with enhanced stability.},
  author={Gawon Kim and Young Hoon Cheon and Hyun Seok Kim},
  journal={Biotechnology and bioengineering},
  year={2000},
  volume={68 2},
  pages={211-7}
}
Bifunctional enzymes find a wide application as a monitoring facility and a potential biocatalyst in molecular biology and biotechnology. Recombination of natural enzymes to a bifunctional fusion offers valuable tools, but the functional and structural instability of artificial fusion enzymes remains to be solved. Based on structural traits of microbial D-hydantoinase, we attempted to construct a bifunctional N-carbamylase/D-hydantoinase fusion enzyme that would be useful for the synthesis of… CONTINUE READING

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  • GJ Kim, HS Kim
  • 1998
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