Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging.

@article{Ai2006DirectedEO,
  title={Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging.},
  author={Hui-wang Ai and J. Nathan Henderson and Stephen J. Remington and Robert E. Campbell},
  journal={The Biochemical journal},
  year={2006},
  volume={400 3},
  pages={531-40}
}
The arsenal of engineered variants of the GFP [green FP (fluorescent protein)] from Aequorea jellyfish provides researchers with a powerful set of tools for use in biochemical and cell biology research. The recent discovery of diverse FPs in Anthozoa coral species has provided protein engineers with an abundance of alternative progenitor FPs from which improved variants that complement or supersede existing Aequorea GFP variants could be derived. Here, we report the engineering of the first… CONTINUE READING

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