Directed evolution of a glutaryl acylase into an adipyl acylase.

@article{Sio2002DirectedEO,
  title={Directed evolution of a glutaryl acylase into an adipyl acylase.},
  author={Charles F. Sio and Anja M Riemens and J C van der Laan and Raymond M D Verhaert and Wim J Quax},
  journal={European journal of biochemistry},
  year={2002},
  volume={269 18},
  pages={4495-504}
}
Semi-synthetic cephalosporin antibiotics belong to the top 10 of most sold drugs, and are produced from 7-aminodesacetoxycephalosporanic acid (7-ADCA). Recently new routes have been developed which allow for the production of adipyl-7-ADCA by a novel fermentation process. To complete the biosynthesis of 7-ADCA a highly active adipyl acylase is needed for deacylation of the adipyl derivative. Such an adipyl acylase can be generated from known glutaryl acylases. The glutaryl acylase of… CONTINUE READING
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