Directed evolution of a biterminal bacterial display scaffold enhances the display of diverse peptides

@article{Rice2008DirectedEO,
  title={Directed evolution of a biterminal bacterial display scaffold enhances the display of diverse peptides},
  author={Jeffrey James Rice and Patrick S. Daugherty},
  journal={Protein Engineering, Design and Selection},
  year={2008},
  volume={21},
  pages={435 - 442}
}
Bacterial cell-surface display systems coupled with quantitative screening methods offer the potential to expand protein engineering capabilities. To more fully exploit this potential, a unique bacterial surface display scaffold was engineered to display peptides more efficiently from the surface exposed C- and N-termini of a circularly permuted outer membrane protein. Using directed evolution, efficient membrane localization of a circularly permuted OmpX (CPX) display scaffold was rescued… CONTINUE READING

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Edited by Andreas Pluekthun J.J.Rice and P.S.Daugherty 442 D ow naded rom http/academ ic.p.com /peds/articlect/21/7/435/1538582 by gest on 08 M arch

  • U. Heinemann, M. Hahn
  • J. Cell Sci.,
  • 1995
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