Directed evolution of Pseudomonas aeruginosa lipase for improved amide-hydrolyzing activity.

Abstract

A lipase from Pseudomonas aeruginosa was subjected to directed molecular evolution for increased amide-hydrolyzing (amidase) activity. A single round of random mutagenesis followed by screening for hydrolytic activity for oleoyl 2-naphthylamide as compared with that for oleoyl 2-naphthyl ester identified five mutants with 1.7-2.0-fold increased relative… (More)

Topics

  • Presentations referencing similar topics