Directed evolution of GFP with non-natural amino acids identifies residues for augmenting and photoswitching fluorescence.

Abstract

Genetic code reprogramming allows proteins to sample new chemistry through the defined and targeted introduction of non-natural amino acids (nAAs). Many useful nAAs are derivatives of the natural aromatic amino acid tyrosine, with the para OH group replaced with useful but often bulkier substituents. Extending residue sampling by directed evolution… (More)
DOI: 10.1039/c4sc02827a

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