DNA polymerases and biotechnological applications.
*Fax: (+1) 858-784-7472, email@example.com, Homepage: http://www.scripps.edu/chem/romesberg. Supporting information for this article is available on the WWW under http://www.angewandte.org or from the author. NIH Public Access Author Manuscript Angew Chem Int Ed Engl. Author manuscript; available in PMC 2011 August 9. Published in final edited form as: Angew Chem Int Ed Engl. 2010 August 9; 49(34): 5921–5924. doi:10.1002/anie.201001607. N IH PA Athor M anscript N IH PA Athor M anscript N IH PA Athor M anscript We present the application of an activity-based phage display method to identify DNA polymerases tailored for next generation sequencing applications. Using this approach, we identify a mutant of Taq DNA polymerase that incorporates the fluorophore-labeled dA, dT, dC, and dG substrates ~50 to 400-fold more efficiently into scarred primers in solution and that also demonstrates significantly improved performance under actual sequencing conditions.