Directed evolution approach to a structural genomics project: Rv2002 from Mycobacterium tuberculosis.

@article{Yang2003DirectedEA,
  title={Directed evolution approach to a structural genomics project: Rv2002 from Mycobacterium tuberculosis.},
  author={Jin Kuk Yang and Min Sik Park and Geoffrey Waldo and Se Won Suh},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2003},
  volume={100 2},
  pages={455-60}
}
One of the serious bottlenecks in structural genomics projects is overexpression of the target proteins in soluble form. We have applied the directed evolution technique and prepared soluble mutants of the Mycobacterium tuberculosis Rv2002 gene product, the wild type of which had been expressed as inclusion bodies in Escherichia coli. A triple mutant I6TV47MT69K (Rv2002-M3) was chosen for structural and functional characterizations. Enzymatic assays indicate that the Rv2002-M3 protein has a… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 18 extracted citations

References

Publications referenced by this paper.
Showing 1-2 of 2 references

Collaborative Computational Project , Number 4 ( 1994 )

  • J. K. Yang, H.-J. Yoon, +5 authors S. W. Suh
  • Acta Crystallogr . D
  • 2002

Structure (London) 8, 339–347

  • J. Obeid, P. C. White
  • Chem. Biol. Interact
  • 1992

Similar Papers

Loading similar papers…