Directed evolution and the creation of enantioselective biocatalysts

@article{Jaeger2001DirectedEA,
  title={Directed evolution and the creation of enantioselective biocatalysts},
  author={K Jaeger and Thorsten Eggert and Andreas Eipper and Manfred T Reetz},
  journal={Applied Microbiology and Biotechnology},
  year={2001},
  volume={55},
  pages={519-530}
}
Directed evolution has emerged as a key technology to generate enzymes with new or improved properties that are of major importance to the biotechnology industry. A directed evolution approach starts with the identification of a target enzyme to be optimized and the cloning of the corresponding gene. An efficient expression system is needed before the target gene is subjected to random mutagenesis and/or in vitro recombination, thereby creating molecular diversity. Subsequently, improved enzyme… CONTINUE READING

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