Directed evolution: tailoring biocatalysts for industrial applications

@article{Kumar2013DirectedET,
  title={Directed evolution: tailoring biocatalysts for industrial applications},
  author={Ashwani Kumar and Suren Singh},
  journal={Critical Reviews in Biotechnology},
  year={2013},
  volume={33},
  pages={365 - 378}
}
Current challenges and promises of white biotechnology encourage protein engineers to use a directed evolution approach to generate novel and useful biocatalysts for various sets of applications. Different methods of enzyme engineering have been used in the past in an attempt to produce enzymes with improved functions and properties. Recent advancement in the field of random mutagenesis, screening, selection and computational design increased the versatility and the rapid development of enzymes… Expand

Topics from this paper

A roadmap to directed enzyme evolution and screening systems for biotechnological applications.
TLDR
Challenges of designing and performing a directed enzyme evolution campaign, current advances in methods, as well as highlighting some examples of its applications in industrially relevant enzymes are presented. Expand
Recent Trends for Discovery and Enhancement of Enzyme Function: A Review
TLDR
State-of-the-art strategies and tools for novel enzyme discovery and function enhancement, including enzyme engineering at the molecular level through different approaches such as directed evolution, rational design, semi rational design and computational de novo enzyme design are highlighted; in addition to the recently developed field, nanobiocatalysis. Expand
Biocatalysts: application and engineering for industrial purposes
TLDR
The present review seeks to highlight the major fields of enzyme application and to provide an updated overview on previous protein engineering studies wherein natural enzymes were modified to meet the operational conditions required for industrial application. Expand
Evolutionary Methods for Improving the Production of Biorenewable Fuels and Chemicals
Evolutionary methods to improve strain performance are a powerful complement to rational, predictive strain engineering. These evolutionary methods can consist of serial transfers or engineeredExpand
Integrated (Meta) Genomic and Synthetic Biology Approaches to Develop New Biocatalysts
TLDR
How the explosion in genomics based sequence analysis, allied with parallel developments in synthetic and molecular biology, have the potential to fast-track the discovery and subsequent improvement of a new generation of marine biocatalysts is discussed. Expand
Engineering Novel and Improved Biocatalysts by Cell Surface Display.
TLDR
This review will introduce the molecular and cellular principles of cell surface display and discuss how it has been applied to engineer enzymes with improved properties as well as to develop surface-engineered microbes as whole-cell biocatalysts. Expand
Enzyme engineering and its industrial applications.
TLDR
This review features engineered enzymes that have been applied in food, animal feed, pharmaceuticals, medical applications, bioremediation, biofuels, and detergents. Expand
Developments and Diversity of Proteins and Enzymes
TLDR
This chapter discusses the strides in the protein/enzyme production and their biotechnological perspectives along with the limitations in scale-up. Expand
Enzymes and Phytohormones from Micromonospora
TLDR
The ongoing development of enzyme biotechnology aids in the improve‐ ment of the industrial biocatalysis field and will lead to the improvement of different industrial purposes such as food, chemicals, textiles, leather, pharmaceuticals, and so on. Expand
Replacing antibodies: engineering new binding proteins.
TLDR
This review aims to capture recent advances in the engineering of nonimmunoglobulin scaffolds as well as some of the applications for these molecular recognition elements in the biomedical field. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 156 REFERENCES
Directed evolution: selecting today's biocatalysts.
TLDR
This review deals with new mutagenesis and recombination strategies published recently including the beta-lactam acylases and a full overview of new methods for creating more specialised mutant libraries is given. Expand
Strategy and success for the directed evolution of enzymes.
  • P. Dalby
  • Biology, Medicine
  • Current opinion in structural biology
  • 2011
TLDR
The combined use of several strategies is likely to be required in practice to improve multiple target properties of an enzyme, as successfully shown by a recent industrial example. Expand
Directed evolution and the creation of enantioselective biocatalysts
TLDR
This work discusses recent examples of creating enantioselective biocatalysts by directed evolution and identifies improved enzyme variants identified, preferably after being secreted into culture medium, by screening or selection for the desired property. Expand
Progress in strategies for sequence diversity library creation for directed evolution
TLDR
This work has shown that combining computational/rational design with directed evolution has been developed as more available means to redesign enzymes and will continue to be developed. Expand
Directed evolution of enzyme stability.
TLDR
Direct evolution has contributed to the development of stable enzymes and to new insight into the principles of protein stability, and several recent examples show that directed evolution is an effective strategy to obtain stable enzymes, especially when used in combination with rational or semi-rational engineering strategies. Expand
Directed Evolution and Biocatalysis.
TLDR
It is suggested that the use of biological catalysis is about to expand dramatically because of the recent developments in the artificial evolution of genes that code for enzymes. Expand
Directed evolution of enzymes for biocatalysis and the life sciences
TLDR
Current enzyme engineers are still learning which combinations of selection methods and techniques for mutagenesis and DNA recombination are most efficient, and deciding where to introduce mutations or where to allow recombination is actively being investigated by combining experimental and computational methods. Expand
Directed evolution of single proteins, metabolic pathways, and viruses.
TLDR
The application of molecular evolution to the design of nucleic acids, which indeed was the first application of in vitro evolution, will not be the subject of this article as it has been reviewed elsewhere. Expand
Recent advances in biocatalysis by directed enzyme evolution.
TLDR
This review summarizes recent advances in applying directed evolution approaches to alter various enzyme properties such as activity, selectivity, substrate specificity, stability, and solubility. Expand
Recent progress in biocatalyst discovery and optimization.
TLDR
The use of enzymes in industrial catalysis continues to grow because of the considerable advantages of natural catalytic systems, but the fitness of an individual high-throughput screen requires an intelligent, process-targeted assay amenable to a chosen screening platform. Expand
...
1
2
3
4
5
...