Directed Evolution of Split APEX2 Peroxidase.

  title={Directed Evolution of Split APEX2 Peroxidase.},
  author={Yisu Han and Tess C. Branon and Jeffrey D. Martell and Daniela Boassa and David Michael Shechner and Mark H. Ellisman and Alice Y Ting},
  journal={ACS chemical biology},
  volume={14 4},
APEX is an engineered peroxidase that catalyzes the oxidation of a wide range of substrates, facilitating its use in a variety of applications from subcellular staining for electron microscopy to proximity biotinylation for spatial proteomics and transcriptomics. To further advance the capabilities of APEX, we used directed evolution to engineer a split APEX tool (sAPEX). A total of 20 rounds of fluorescence activated cell sorting (FACS)-based selections from yeast-displayed fragment libraries… CONTINUE READING
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