• Biology, Medicine
  • Published in Nature Biotechnology 2001
  • DOI:10.1038/90831

Direct visualization of protein interactions in plant cells

@article{Subramaniam2001DirectVO,
  title={Direct visualization of protein interactions in plant cells},
  author={Rajagopal Subramaniam and Darrell Desveaux and C Spickler and Stephen W. Michnick and Normand Brisson},
  journal={Nature Biotechnology},
  year={2001},
  volume={19},
  pages={769-772}
}
The protein NPR1/NIM1 is required for the induction of systemic acquired resistance (SAR) in plants and has been shown to interact with members of the TGA/OBF family of basic leucine zipper (bZIP) transcription factors. However, to date, there is no method available to monitor such interactions in plant cells. We report here an in vivo protein fragment complementation assay (PCA), based on association of reconstituted murine dihydrofolate reductase (mDHFR) with a fluorescent probe to detect… CONTINUE READING

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