Direct visualization of Agrobacterium-delivered VirE2 in recipient cells

@inproceedings{Li2014DirectVO,
  title={Direct visualization of Agrobacterium-delivered VirE2 in recipient cells},
  author={Xiaoyang Li and Qinghua Yang and Haitao Tu and Zijie Lim and Shen Pan},
  booktitle={The Plant journal : for cell and molecular biology},
  year={2014}
}
Agrobacterium tumefaciens is a natural genetic engineer widely used to deliver DNA into various recipients, including plant, yeast and fungal cells. The bacterium can transfer single-stranded DNA molecules (T-DNAs) and bacterial virulence proteins, including VirE2. However, neither the DNA nor the protein molecules have ever been directly visualized after the delivery. In this report, we adopted a split-GFP approach: the small GFP fragment (GFP11) was inserted into VirE2 at a permissive site to… CONTINUE READING
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