Direct spectrophotometry of total bile acids in serum.

@article{Mashige1981DirectSO,
  title={Direct spectrophotometry of total bile acids in serum.},
  author={Fumiko Mashige and Naomi Tanaka and Anton Maki and Satomi Kamei and Masaru Yamanaka},
  journal={Clinical chemistry},
  year={1981},
  volume={27 8},
  pages={1352-6}
}
In this simple and direct method for determining total bile acids in serum, the serum was mixed with sodium pyruvate, a lactate dehydrogenase blocker, and bile acids were then measured spectrophotometrically after the following enzyme reaction. Bile acids are converted to 3-oxo bile acids with 3 alpha-hydroxysteroid dehydrogenase (EC 1.1.1.50) with concomitant reduction of NAD+ to NADH. The hydrogen in the NADH generated is transferred by diaphorase (EC 1.6.4.3) to nitrotetrazolium blue to… CONTINUE READING

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