Direct spectrophotometric detection of cation flux in membrane vesicles: stopped-flow measurements of acetylcholine-receptor-mediated ion flux.

@article{Karpen1983DirectSD,
  title={Direct spectrophotometric detection of cation flux in membrane vesicles: stopped-flow measurements of acetylcholine-receptor-mediated ion flux.},
  author={Jeffrey W. Karpen and Alan B. Sachs and Derek J. Cash and Elena B. Pasquale and George P. Hess},
  journal={Analytical biochemistry},
  year={1983},
  volume={135 1},
  pages={
          83-94
        }
}
The development of a spectrophotometric stopped-flow method to measure ion flux in membrane vesicles in the millisecond to minute time region is described in detail. The technique is based on fluorescence quenching of an entrapped fluorophore (anthracene-1,5-disulfonic acid) by Cs+. The method has been applied to the measurement of acetylcholine-receptor-mediated ion flux in membrane vesicles prepared from the electric organs of both Electrophorus electricus and Torpedo californica. The method… Expand
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The construction and use of a versatile quenched flow machine suitable for use with small volumes of membrane vesicle suspension with a wide range of incubation times is described, exposing the varying response to different ligands, and the different ligand dependencies of ion flux and inactivation of receptor. Expand
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